Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPWLYWTLKPKHRIWAEEWQKEYQAYLMDMETVEIGENCFISPLAHIFAEPGRKIKIGDNCFI-AADCSLHGP--LEIGNEVAINHHCILDGGRAGIKLHDQVRIAAYCHLYAFDHGMQLDRPLYQQPVRSQGIEIEKDVWLGAHVGIKDGIKIGKHAVVGMNSMVTKDV-EPYHIVGGNPAKFIRLRE 2WLF Chain:B ((33-192)) ----------------------------NNNSVYIGNNCKIVSSNIRLKGNNITLFIADDVEIMGLVCSLHSDCSLQIQAKTTMGNGEITIAEKGKISIGKDCMLAHGYEIRNTDMHPIYSLENGERINHGKDVIIGNHVWLGRNVTILKGVCIPNNVVVGSHTVLYKSFKEPNCVIAGSPAKIVKEN-

General information: TITO was launched using: RESULT: Template: 2WLF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 863 -15408 -17.85 -98.77 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : -17.85 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.405

(partial model without unconserved sides chains): PDB file : Tito_2WLF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WLF-query.scw PDB file : Tito_Scwrl_2WLF.pdb: