Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACVAGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFGPLVVDPVIVTKHGNLLLEES-ALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFVQAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFES-GKSFWLNAPFYPTDRINGTGDSLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE 4JJP Chain:A ((8-248)) ----KIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAITAQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPKYLVVDPVMISKSGYYLLKPEAKENLI-KYLIPLAYIITPNIPEAEEITGIKIHNVDDMKRVGEEILQLGPKFVLMKGGHLDG-EAV----DI-LVGKNIFKVYKSE----------GCTLSSAITSYLALGYEITEAVNLSKIYITEAIK---------------------

General information: TITO was launched using: RESULT: Template: 4JJP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1302 -72779 -55.90 -317.81 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -55.90 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_4JJP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JJP-query.scw PDB file : Tito_Scwrl_4JJP.pdb: