Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTESTTPKFAIPELPMQIQTIRQYLPHRYPFLLVDRVTEVTDN-SIIGYKNVSINEEFLQGHFPEYPIMPGVLIVEALAQVSGVLGFIMNNETPKPGSLFLFAGAERVRFKKQVVAGDQLVLKSELVMQKR--GIYKYNCTASVDGIVAATAEIMISHQKTEQA 3AZ9 Chain:D ((11-141)) ---------------IDIEDIKKILPHRYPFLLVDKVIYMQPNKTIIGLKQVSTNEPFFNGHFPQKQIMPGVLQIEALAQLAGIL--CLKSDDSQKNNLFLFAGVDGVRWKKPVLPGDTLTMQANLISFKSSLGIAKLSGVGYVNGKV----------------

General information: TITO was launched using: RESULT: Template: 3AZ9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 524 -46145 -88.06 -360.51 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain D : 0.79 3D Compatibility (PKB) : -88.06 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.634

(partial model without unconserved sides chains): PDB file : Tito_3AZ9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3AZ9-query.scw PDB file : Tito_Scwrl_3AZ9.pdb: