Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNILIANDDGVFAPGIQALADALKPLGRVVVVAPESERSGFSSALTLDRPLRPIQIAEDVWAV-NGTPADCVYLSMNGLFDFEFDLVVSGINRGANLGDDVLYSGTVGAAFEGRLMKQPAIAVSLAGPDVRSYDHKDDYAQAAKWVHDFITKGLP-ALPPRHIFNINIPDVP--QLKGTQITYQGRRAQSKPITSHVDPRGRQVYWIGLAGEAVTDPQRIASQIQSDFFAVANGFVSVTPIQMDATNYAVLEDLQASLG 2V4N Chain:A ((2-245)) MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNSLTLESSLRTFTFDNGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVIYSGTVAAAMEGRHLGFPALAVSLNGYQ------H--YDTAAAVTCALLRGLSREPLRTGRILNVNVPDLPLAQVKGIRVTRCGSRHPADKVIPQEDPRGNTLYWIGPP--G----DKYDAGPDTDFAAVDEGYVSVTPLHVDLTAHSAHDVVSDWL-

General information: TITO was launched using: RESULT: Template: 2V4N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1361 -60396 -44.38 -251.65 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -44.38 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.443

(partial model without unconserved sides chains): PDB file : Tito_2V4N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2V4N-query.scw PDB file : Tito_Scwrl_2V4N.pdb: