Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILITGANGQLGTELRYLLDERNE-EYVAVDVAEMDITDAEMVEKVFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNVAKASEKHGATLVYISTDYVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKNFVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDTTWYDFAVEILKDTDVEV--KPVDSSQFPAKAKRPLNSTMSLAKAKA-TGFVIPTWQDALQEFYKQEVR 1VL0 Chain:C ((14-291)) KILITGANGQLGREIQKQLKGK-NVEVIPTDVQDLDITNVLAVNKFFNEKKPNVVINCAAHTAVDKCEE-QYDLAYKINAIGPKNLAAAAYSVGAEIVQISTDYVFDGEA--KEPITEFDEVNPQSAYGKTKLEGENFVKALNPKYYIVRTAWLYGDGN-NFVKTMINLGKTHDELKVVHDQVGTPTSTVDLARVVLKVIDEK-NYGTFHCTCKG--ICSWYDFAVEIFRLTGIDVKVTPCTTEEFPRPAKRPKYSVLRNYMLELTTGDITREWKESLKEYIDLLQ-

General information: TITO was launched using: RESULT: Template: 1VL0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1623 1763 1.09 6.43 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain C : 0.79 3D Compatibility (PKB) : 1.09 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.465

(partial model without unconserved sides chains): PDB file : Tito_1VL0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VL0-query.scw PDB file : Tito_Scwrl_1VL0.pdb: