Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLVTAIVKPFKLDDVREALSDIGVQGITVTEVKGFGRQKGHTELYRGAEYVVDFLPKVKIEIAISDEMVDAVIESITRVASTGKIGDGKIFLTNLEQVIRIRTGETGPDAV 2J9C Chain:C ((3-114)) MKKVEAIIRPEKLEIVKKALSDAGYVGMTVSEVKGRGVQGGIVERYRGREYIVDLIPKVKIELVVKEEDVDNVIDIICENARTGNPGDGKIFVIPVERVVRVRTKEEGKEAL

General information: TITO was launched using: RESULT: Template: 2J9C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 430 -54013 -125.61 -482.25 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain C : 0.84 3D Compatibility (PKB) : -125.61 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.509

(partial model without unconserved sides chains): PDB file : Tito_2J9C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2J9C-query.scw PDB file : Tito_Scwrl_2J9C.pdb: