Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIISKDIKMLALVTGASAGFGYSISKKLIESGYNVIGCGRRAEKLEELQKQLGENFYPLVFDMTDTAENINKLFKELPNEFQIDQIDLLVNNAGLALGLEPADKADLDDWYTMIDTNVKGFVTVTRLILPSMVKKKSGLIINMGSIAGTYPYPGGNVYGATKAFVEQFSLNLRADLAGTGVRVTNIEPGLCGGTEFSLVRFKGDQEKANSLYDKKNPILPEDIANTVAWIASQPPHININRIEMMPTTQTFNPLKVVEVE 3ASU Chain:B ((1-240)) --------MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDELGDNLYIAQLDVRNRAA-IEEMLASLPAEWC--NIDILVNNAGLALGMEPAHKASVEDWETMIDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLNLRTDLHGTAVRVTDIEPGLV------------------------VALTPEDVSEAVWWVSTLPAHVNINTLEMMPVTQSY---------

General information: TITO was launched using: RESULT: Template: 3ASU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1147 -127081 -110.79 -588.34 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -110.79 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.592

(partial model without unconserved sides chains): PDB file : Tito_3ASU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ASU-query.scw PDB file : Tito_Scwrl_3ASU.pdb: