Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITVSRKKRGSKKMKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPEKFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFYRDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVKKWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEIGIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMPEIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKK---MNPYRGWEIYEKGIYDILTNTRENYGN-IKCYISENGMGVEGEERFVNSEGIIEDDYRIEFVTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKSGRWFKTVSEKNGFDA 5GNZ Chain:K ((11-453)) ---------------SYRFPEGFLWGAATAAYQIEGSSMADGAGESIWDR-FSHTPGNMKDGDTGDVACDHYNRWREDIELMKRLNLQAYRFSVSWSRVIPQGRGAINPKGLAFYDRLVDGLLEAGIEPLATLYHWDLPAALDDRGGWLNPDIADWFADYGQVLFEKFKGRVKTWGTINEPWCIVDGGYLHGALAPGHRSAYEAVIAGHNVLRAHGAAVRRFREVG-EGQIGIVLNIEPKYPASD-KPEDEAARRRAEAQMNRWFLDPLMGRGYPEELTDVYG--AAWREFPKEDFELIAEPT-DWMGLNWY------TRAVP---ENAP---DAWPTRSRPVRQTQHAHTETGWEVYPPALTDTLVWLSEQTGGKLPLMVTENGSAWYDPPHAI--DGRIHDPMRVHYLQTHIKALHDAIGKGVDLRGYMVWSLLDNLEWSLGYSKRFGIVHVNFATQ-ERTIKDSGLFYAEVIKTHG---

General information: TITO was launched using: RESULT: Template: 5GNZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain K - contact count / total energy / energy per contact / energy per residue : 2645 -129680 -49.03 -295.40 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain K : 0.78 3D Compatibility (PKB) : -49.03 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.445

(partial model without unconserved sides chains): PDB file : Tito_5GNZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5GNZ-query.scw PDB file : Tito_Scwrl_5GNZ.pdb: