Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIRGFELVSSFTDEN-LLPKRETAHAAGYDLKVAVRTVVAPGEIVLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQMKNITD-QEVVLEVGERIVQAVFATFLIADGDAAD----------GVRT-GGFGSTGH 3HZA Chain:A ((21-170)) ---MSTTLAIVRLDPGLPLPSRAHDGDAGVDLYSAEDVELAPGRRALVRTGVAVAVPFGMVGLVHPRSGLATRVGLSIVNSPGTIDAGYR------GEIKVALINLDPAAPIVVHRGDRIAQLLVQRVELVE-LVEVSSFDEAGLASTSRGDGGWGSSGG

General information: TITO was launched using: RESULT: Template: 3HZA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 579 -27168 -46.92 -198.31 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -46.92 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.413

(partial model without unconserved sides chains): PDB file : Tito_3HZA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HZA-query.scw PDB file : Tito_Scwrl_3HZA.pdb: