Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVVVDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKVLGCEGVIVTNAAGGIG--FGPGTLMAISDHINM---TGQNPLMGENLDDFGPRFPDMSRAYTPEYRATAHEVAKKL--NIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVAAHSGLKVLGISCITNFAAGFQEEL---NHEEVVEVTERVKGDFKGLLKAILAEL 2A0X Chain:A ((13-282)) ---------TAEWLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYSEIPNFPRSTVPGHAGRLVFGFLNGRACVMMQGRFHMYEGYPLWKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTWKQMGEQRELQEGTYVMVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLEKANFEEVLAAGKQAAQKLEQFVSILMASI

General information: TITO was launched using: RESULT: Template: 2A0X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1401 -88449 -63.13 -340.19 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -63.13 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.582

(partial model without unconserved sides chains): PDB file : Tito_2A0X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2A0X-query.scw PDB file : Tito_Scwrl_2A0X.pdb: