Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILKIEDLVMSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKVSLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLLTCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGYNHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGADIIMLDNMS-LEQIEQAITLIAGRSRI--ECSGNIDMTTISRFRGLAIDYVSSGSLTHSAKSLDFSMKGLTYLDV 2B7P Chain:C ((2-271)) --------------------EIRTFLERALKEDLGHGDLFER-VLEKDFKATAFVRAKQEGVFSGEKYALELLEMTGIECV----QTIKDKERFKPKDALMEIRGDFSMLLKVERTLLNLLQHSSGIATLTSRFVEALNSHKVRLLDTRKTRPLLRIFEKYSVLNGGASNHRLGLDDALMLKDTHLRHVKDLKSFLTHARKNLPFTAKIEIECESFEEAKNAMNAGADIVMCDNLSVLETKEIAAYRDAHYPFVLLEASGNISLESINAYAKSGVDAISVGALIHQATFIDMHMK-------

General information: TITO was launched using: RESULT: Template: 2B7P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1329 -109298 -82.24 -409.36 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain C : 0.75 3D Compatibility (PKB) : -82.24 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.446

(partial model without unconserved sides chains): PDB file : Tito_2B7P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2B7P-query.scw PDB file : Tito_Scwrl_2B7P.pdb: