Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQVDIAALSPQVVWQHFQTLCTIPRPSKHEQQLREFLQNWAESRNLETYVDEVGNLIIRKNATPGKKHVSGVILQGHLDMVTQANTGTVHDFFKDPIRPVLEEGWLIAKDTTLGADNGIGVALALAVLDSND---IHGPIEVLLTVDEEAG-MSGARLLETGVLKGKWLFNIDTEEWGELYLGCAGSIDVEVEQPLNYEPIPENLNIVNIQVAGLKGGHSGVDIHLGRGNANVILARFLNQHLAS-----------LGGHLVEFTGGTARNALPREAVATIAISPNQLSSLEKLLAEYQAAWKEQLKGIDDNLQLSIQSTGAKVTEVINQQQQNEWLQALATSPYGVASMSQVLPDVVETSNNIGVVRLNREGGKAILMVRSMVNQEAQDFAEKIQAHFSQFNIGSTLTPLVSGWTPNPDSAALKCLQQAYQNAFNIEPNLKVIHAGLECGIIAEHYPHLQMVSFGPDI-QGAHAPGERVKVDTVEKCWKLLVTALASVE 3CT9 Chain:B ((7-354)) -------PTMTAEAVSLLKSLISIPSISREETQAADFLQNYIEAEGMQTG-RKGNNVWCLSPMFD----KPTILLNSHIDTVKPV---------KDPFTPREENGKLYGLGS---NDAGASVVSLLQVFLQLCRTSQNYNLIYLASCEEEVSGKEGIESVLPGLPPVSFAIVGEPTE-MQPAIAE------------------KGLMVLDVTATGK-AGHAARD---EGDNAIYKVLNDIAWFRDYRFEKESPLLGPVKMSVTVINAGTQHNV----------------------------------------------------------------------------------------------VP---DKCTFVVDIRSNELYSNEDLFAEIRKHIACDA-KAR-SFRLNSSRIDEKHPFVQKAVKMGR-------IPFGSPTLSDQALMS-----FASVKIGPGRSSRSHTAEEYIMLKEIEEAIGIYLDLLDGL-

General information: TITO was launched using: RESULT: Template: 3CT9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1723 4777 2.77 14.65 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : 2.77 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.377

(partial model without unconserved sides chains): PDB file : Tito_3CT9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CT9-query.scw PDB file : Tito_Scwrl_3CT9.pdb: