Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENDMKKISLVIAASTMSLSVFAAAPITNKSPAKDQFSYSYGYLMGRNNTDALTDLNLDIFYQGLQEGAQNKTARLTDEEMAKAINDYKKTLEAKQLVEFQKQGQQNAQAGAAFLAENTKKSGVITTKSGLQYQVLK---EGTGKTPKATSRVKVNYEGRLLD-GTVFDSSIARNHPVDFQLNQ--VIAGWTKGLQTMKEGGKTRFFIPAKLAYGEVGAGDSIGPNSTLIFDIELLQVLPK 4J4N Chain:A ((12-123)) ----------------------------------------------------------------------------------------------------------------------------LTADGGVIKTILKKGDEGEENIPKKGNEVTVHYVGKLESTGKVFDSSFDRNVPFKFHLEQGEVIKGWDICVSSMRKNEKCLVRIESMYGYGDEGCGESIPGNSVLLFEIELL-----

General information: TITO was launched using: RESULT: Template: 4J4N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 435 8118 18.66 76.58 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 18.66 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.413

(partial model without unconserved sides chains): PDB file : Tito_4J4N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J4N-query.scw PDB file : Tito_Scwrl_4J4N.pdb: