Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQSVCILGVTGSIGQSTLKILGQHPDKYSVFAVSAH-SRISELVEICKQFRPKVVVVPEQKIAELKTLFAQQNISAIDVLAGQEGLVDIASHTDVDIVMAAIVGAAGLLPTLAAVKAGKRVLLANKEALVMSGEIMMQAARDHQALLLPVDSEHNAIFQSLPHNYLQADRTGQPQLGVSKILLTASGGPFLNHSLEQLVHVTPQKACKHPNWSMGQKISVDSATLMNKGLELIEACHLFSISEHFVTVVVHPQSIIHSMVQYVDGSTLAQMGNPDMCTPIAHALAWPERLQTNVPALDLFEYSQLNFQAPDTQKFPALNLARQAMRAGGLAPTILNAANEIAVEAFLMERIGFTSIPQVVEHTLEKLENTAAE--SIECILDKDKVARSVAQQYIKYWRLK 4RCV Chain:A ((30-402)) ----VVVLGSTGSIGTQALQVIADNPDRFEVVGLAAGGAHLDTLLRQRAQTGVTNIAVADEH--------AAQRVGDIP-YHGSDAATRLVEQTEADVVLNALVGALGLRPTLAALKTGARLALANKESLVAGGSLVLRAARPGQ--IVPVDSEHSALAQCL--------RGGTPDE-VAKLVLTASGGPFRGWSAADLEHVTPEQAG--------PMNTLNSASLVNKGLEVIETHLLFGIPYDRIDVVVHPQSIIHSMVTFIDGSTIAQASPPDMKLPISLALGWPRRVSGAAAACDFHTASSWEFEPLDTDVFPAVELARQAGVAGGCMTAVYNAANEEAAAAFLAGRIGFPAIVGIIADVLHAADQWAVEPATVDDVLDAQRWARERAQRAVS-----

General information: TITO was launched using: RESULT: Template: 4RCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2151 -125023 -58.12 -345.37 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -58.12 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.477

(partial model without unconserved sides chains): PDB file : Tito_4RCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RCV-query.scw PDB file : Tito_Scwrl_4RCV.pdb: