Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKQSAGQLFRDAVAQEKPLQVVGTINANHALLAKRAGYKAIYLSGGGVAAGSLGLPDLGISNLDDVLTDVRRITDVCDLPLLVDADTGFGASAFNIARTTKALIKFGAAAMHIEDQVGAKRCGHRPNKAIVTQEEMVDRIKAAVDARGDDSFVIMARTDALAVEGLQAAIDRAGAYIEAGADMLFPEAITELDMYKQFAQKTGVPILANITEFGSTPLFTTEELASADVSLALYPLSAFRAMNKAAETVYETLRKEGTQKNVVDIMQTRKELYERINYYAFEDYLDNAFAKKK 3EOO Chain:N ((6-292)) --LISAGAKFRAAVAAEQPLQVVGAITAYAAKMAEAVGFKAVYLSGGGVAANSLGIPDLGISTMDDVLVDANRITNATNLPLLVDIDTGWGG-AFNIARTIRSFIKAGVGAVHLEDQVGQKRCGHRPGKECVPAGEMVDRIKAAVDARTDETFVIMARTDAAAAEGIDAAIERAIAYVEAGADMIFPEAMKTLDDYRRFKEAVKVPILANLTEFGSTPLFTLDELKGANVDIALYCCGAYRAMNKAALNFYETVRRDGTQKAAVPTMQTRAQLYDYLGYYAYEEKLDQLF----

General information: TITO was launched using: RESULT: Template: 3EOO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain N - contact count / total energy / energy per contact / energy per residue : 1367 -159914 -116.98 -557.19 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain N : 0.89 3D Compatibility (PKB) : -116.98 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.624

(partial model without unconserved sides chains): PDB file : Tito_3EOO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EOO-query.scw PDB file : Tito_Scwrl_3EOO.pdb: