Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFMSQIVDIRAREILDSRGNPTIEADVILESGVVGRACAPSGASTGSREALELRDGDKSRYLGKGVRTAVQNVNSSIHELLVGQSVFEQKALDEKMIAFDGTENKSKLGANATLAVSLAAAHAAAAEQKLPLFQYIANLRGQTTLTMPVPMMNILNGGAHADNTVDIQEFMIEPVGFTSFAEALRAGAEVFHSLKSVLKKQGLNTAVGDEGGFAPNLRSNEEAITVILQAIEQTGYKAGSDIMLALDCASSEFYKNGQYILEGEGNKSFTSNQFADYLAGLVKQYPIISIEDGLDESDWEGWSYLTSILGDKIQLVGDDLFVTNPKILQRGIDEKVGNSILIKYNQIGTLTETLDAIYLAKANGYTTVISHRSGETEDSTIADLAVGTAAGQIKTGSLCRSDRVSKYNQLLRIEELTKAVYRGKAEFKGLK 3QN3 Chain:C ((4-406)) --MLVIEDVRAYEVLDSRGNPTVKAEVTLSDGSVGAAIVPSGASTGSKEALELRDNDE-RFGGKGVLKAVANVNETIADEILGLDAFNQTQLDDTLRELDGTNNYSNLGANATLGVSMATARAAAAALGMPLYRY---LGGANASILPVPMCNIINGGAHANNNVDFQEFMIMPFGFTSFKEALRSVCEIYAILKKELANSGHSTALGDEGGFAPNLANNTEPIDLLMTCIKKAGYE--NRVKIALDVASTEFFKDGKYHMEG---KAFSSEALIERYVELCAKYPICSIEDGLAENDFEGWIKLTEKLGNKIQLVGDDLFVTNEDILREGIIKKMANAVLIKPNQIGTITQTMRTVRLAQRNNYKCVMSHRSGESEDAFIADFAVALNTGQIKTGALARGERTAKYNRLLEIE-----------------

General information: TITO was launched using: RESULT: Template: 3QN3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2550 -163850 -64.25 -406.57 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain C : 0.85 3D Compatibility (PKB) : -64.25 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.513

(partial model without unconserved sides chains): PDB file : Tito_3QN3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QN3-query.scw PDB file : Tito_Scwrl_3QN3.pdb: