Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSLSQVNADVLKQAQQRIQQDLLTTLAAFSIPEPLKSAVHHAVMLGGKRVRPALCYATASLQDNPNFAAARRAAVAVELIHCYSLAHDDLPCMDNDLLRRGQPTCHVAFGEDTALLAGDILQSMAFEVLGSRLFDEQGQGTDAAIVLKQIQILAT--ASSKMVCGQVLDLQAEAK--QISQDELENIHRNKTGALIQAAIMMGAVTIFPGTDQAIPKLRQYGQAIGLAFQVQDDILDITSSTETLGKTAGKDEQVQKSTYPALMGLEQAQIYAKELHDQAFEALAHFGENAKELVEISQFLLARTN 4LLS Chain:A ((24-311)) -------FTQRLDAAAAAVQAHFDKVLAAF-EPLPIVEAMAHAT-SGGKRLRGFLVLETARLH-DIAAGEAIWSATAIEALHAYSLVHDDLPCMDNDDMRRGQPTVHKKWDDATAVLAGDALQTLAFELVTHPGAS-----ASAEVRADLALSLARASGAQGMVLGQALDIAAETARAPLSLDEITRLQQGKTGALIGWSAQAGARL-AQAD---TAALKRYAQALGLAFQIADDILDVTGDSAQVGKAVGKDASAGKATFVSLLGLDAARARAMSLIDEACDSLATYGAKADTLRETARFVVRRTH

General information: TITO was launched using: RESULT: Template: 4LLS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1440 -99386 -69.02 -349.95 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -69.02 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.492

(partial model without unconserved sides chains): PDB file : Tito_4LLS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LLS-query.scw PDB file : Tito_Scwrl_4LLS.pdb: