Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNVEKLGGKTMTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPDLMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSIVQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQLG 3OOW Chain:F ((5-164)) -----------SVQVGVIMGSKSDWSTMKECCDILDNLGIGYECEVVSAHRTPDKMFDYAETAKERGLKVIIAGAGGAAHLPGMVAAKTTLPVLGVPVKSSTLNGQDSLLSIVQMPAGIPVATFAIGMAGAKNAALFAASILQHTDINIAKALAEFRAEQTRFVLENPDPR-

General information: TITO was launched using: RESULT: Template: 3OOW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 813 -116651 -143.48 -729.07 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain F : 0.86 3D Compatibility (PKB) : -143.48 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.628

(partial model without unconserved sides chains): PDB file : Tito_3OOW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OOW-query.scw PDB file : Tito_Scwrl_3OOW.pdb: