Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISKKSKDKEELKMTAIYNSVTELIGKTPIVKLNKIVPEDSA--DVFVKLEFFNPGGSVKDRIALSMIEKAEHDGLLKPGDTI-IEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIERRLLMKGYGAELILTPGADGISGSIREAERLAKE-NGYFLPLQFENEANPLVHEKTTGPEIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKSRINRGRTSRICYFRRERSGTSQNPRNRYRFCS 4LMA Chain:A ((10-174)) ----------------IAHDVTELVGRTPLVQLNRIPQAEGCLGRIVMKLEGMNPAASVKDRIGTHMINSAEKAGLINPETTVLVEPTSGNTGIALAMTAAAKGYRLILTMPETMSLERRAMLKAYGATLELTPGSQGMKGAILRAQQIVDSIPGAYMLQQFRNPSNPEIHRLTTAEEIWQ------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4LMA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 728 22846 31.38 141.90 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : 31.38 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.407

(partial model without unconserved sides chains): PDB file : Tito_4LMA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LMA-query.scw PDB file : Tito_Scwrl_4LMA.pdb: