Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLQNPKKFKMMIDQAHDVAINVLRPISRKYDKAEHAYPKELDMLASLIDGMNEGGEGINAGAAGAGKRGESDNEGVRNGTNMSTALGIIEMCYGDTGLLLSMPRQGLGNSAIAAVANDEQLERF------KGTWAAMAITEPGCGSDSAAIRTTATKDGNDYILNGEKIFVTSGERADSVVVWATLDRKL---GRAAIKSFVVPKGTPGMKVERLEHKLGIKASDTAVISFTDCRVPAENLLGNPEIDVAKGFAGVMETFDNTRPLVAAMAIGCAKASLERIKEIFKDQLDSNYSTPYLQTSNLAAQIYRMEAEWEAARLLMLKATWMADNKKPNSKEASIAKAKAGRVGNEITLKCVELAASVGYKEDELLEKWARDSKILDIFEGTQQIQQLIIARRELGKSSSELK 4P13 Chain:D ((3-386)) FSFEFTEQQKEFQATARKFAREEIIPVAAEYDKTG-EYPVP--LIRRAWELGLM---N-------T---HIPENCGGLGLGTFDACLISEELAYGCTGVQTAIEGNSLGQMPIIIAGNDQQKKKYLGRMTEEPLMCAYCVTEPGAGSDVAGIKTKAEKKGDEYIINGQKMWITNGGKANWYFLLARSDPDPKAPANKAFTGFIVEADTPGIQIGRKELNMGQRCSDTRGIVFEDVKVPKENVLIGD----GAGFKVAMGAFDKTRPVVAAGAVGLAQRALDEATKYALE--RKTFGKLLVEHQAISFMLAEMAMEVELARMSYQRAAWEVDSGRRNTYYASIAKAFAGDIANQLATDAVQILGGNGFNTEYPVEKLMRDAKIYQIYEGTSQIQRLIVAREHIDKYK----

General information: TITO was launched using: RESULT: Template: 4P13.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2199 71027 32.30 189.40 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain D : 0.79 3D Compatibility (PKB) : 32.30 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.475

(partial model without unconserved sides chains): PDB file : Tito_4P13.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4P13-query.scw PDB file : Tito_Scwrl_4P13.pdb: