Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVTSVNPATNATNEYYLTRQSQMESNVRSYPRKLPLAIAKAQGCWVTDVEGTQYLDCLAGAGTLALGHNHPAVIQSIQDTLASGLPLHTLDLTTPLKDAFTEALLAYLPGGKEEYCLQFCGPSGADATEAAIKLAKTYTGRSSVISFSGGYHGMTHGSLAMTGNLS-AKNAVNGLMPGVQFMPYPHEYRCPLGLGGEAGVDALTYYFENFIEDVES--GVTKPAAVILEAIQGEGGVVTAPVKWLQKIREVTEKHNIVLILDEVQAGFARSGKMFAFEHAGIEPDVVVMSKAVGGGLPLAVLGIKRKFDA-WQPAGHTGTFRGNQLAMGTGLVVLETIKEQNLAQNAQERGEFLQAELKKLATEFPCIGNVRGRGLMIGVEIVDERKPADRIGSHPADSQLAAAIQTACFNNKLLLEKGGRNGTVIRLLCPLIITQEECVEVIARFKKAVAEALVAVRGA 4FFC Chain:A ((46-450)) ----------------------------------APVYAVDADGGVIVDADGNSFIDLGAGIAVTTVGASHPAVAAAIADQATHFTHTCFMVTPYEQYVQVAELLNALTPGD-HDKRTALFN-SGAEAVENAIKVARLATGRPAVVAFDNAYHGRTNLTMALTAKSMPYKSQFGPFAPEVYRMPASYPLRDEPG-------LTGEEAARRAISRIETQIGAQSLAAIIIEPIQGEGGFIVPAPGFLATLTAWASENGVVFIADEVQTGFARTGAWFASEHEGIVPDIVTMAKGIAGGMPLSAVTGRAELMDAVYAGGLGGTYGGNPVTCAAAVAALGVMRELDLPARARAIEASVTSRLSALAEEVDIIGEVRGRGAMLAIEIVKPGTLE---P----DAALTKSIAAEALSQGVLILTCGTFGNVIRLLPPLVIGDDLLDEGITALSDIIRAKA------

General information: TITO was launched using: RESULT: Template: 4FFC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2471 -100904 -40.84 -251.63 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -40.84 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.576

(partial model without unconserved sides chains): PDB file : Tito_4FFC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FFC-query.scw PDB file : Tito_Scwrl_4FFC.pdb: