Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSKANHAKTVICGIINVTPDSFSDGGQFFALEQALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDTWKSQVAEAALAAGADLVNDITGLMGDEKMPHVVAEARAQVVIMFNPVMARPQHPSSLIFPHFGFGQAFTEEELADFETLPIEELMEAFFERALARAAEAGIAPENILLDPGIGFGLTKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTASAHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK 2DZA Chain:B ((24-286)) ----LDLDRVRLLGVLNLTPDS------------ALERAREMVAEGADILDLGAESTRP---PVPVEEEKRRLLPVLEAVLS-LGVPVSVDTRKPEVAEEALKLGAHLLNDVTGLR-DERMVALAARHGVAAVVMHMPVP-DPATMM------A----------HARY-RDVV-AEVKAFLEAQARRALSAGVP--QVVLDPGFGFGKLLEHNLALLRRLDEIVALGHPVLVGLSRKRTIGELSGVE----------DPAQRVHGSVAAHLFAVMKGVRLLRVHDVRAHREALGVWEALY--------------

General information: TITO was launched using: RESULT: Template: 2DZA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1373 -159489 -116.16 -643.10 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -116.16 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.583

(partial model without unconserved sides chains): PDB file : Tito_2DZA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2DZA-query.scw PDB file : Tito_Scwrl_2DZA.pdb: