Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSDPRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAIFKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKFLAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLEVPEVTLIDRFGRLGYD-LYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKELTLLSEKVALNLHQQ-EKAGKIVILKIRYEDFSTLTKRKSIAQKTQDASQISQIALQLYEELSEKERGVRLLGITMTGF 1S0O Chain:B ((3-340)) -------------VLFVDFDYFYAQVEEVLNPSLKGKPVVVCVFSGRFEDSGAVATANYEARKFGVKAGIPIVEAKKILPNAVYLPMRKEVYQQVSSRIMNLLREYSEKIEIASIDEAYLDISDKVRDYREAYNLGLEIKNKILEKEKITVTVGISKNKVFAKIAADMAKPNGIKVIDDEEVKRLIRELDIADVPGIGNITAEKLKKLGINKLVDTLSIEFDKLKGMIGEAKAKYLISLARDEYNEPIRT-RVRKSIGRIVTMKRNSRNLEEIKPYLFRAIEESYYKLDKRIPKAIHVVAVT---EDLDIVSRGRTFPHGISKETAYSESVKLLQKILEEDERKIRRIGVRFSKF

General information: TITO was launched using: RESULT: Template: 1S0O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1635 -134613 -82.33 -400.63 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -82.33 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.532

(partial model without unconserved sides chains): PDB file : Tito_1S0O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1S0O-query.scw PDB file : Tito_Scwrl_1S0O.pdb: