Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEIEASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLIGYNTDGKGFFKCL-P-SFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEKTRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQS-SPVPENIVLPETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIWQSLTEKYQ 2EV9 Chain:B ((1-261)) ------MLRFAVLGHPVAHSLSPAMHAFALESLGLEGSYEAWDTPLEALPGRLKEVR-RAFRGVNLTLPLKEAALAHLDWVSPEAQRIGAVNTVLQVEGRLFGFNTDAPGFLEALKAGGIPLK-GPALVLGAGGAGRAVAFALREAGL-EVWVWNRTPQRA--LALAEEFGLR---------AVP----LEKAREARLLVNATRVGLEDPSASPL-PAELFPEEGAAVDLVYRPLWTRFLREAKAKGLKVQTGLPMLAWQGALAFRLWTGLLPDPSGMEEAARRAL-

General information: TITO was launched using: RESULT: Template: 2EV9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1568 -87402 -55.74 -338.77 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -55.74 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_2EV9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EV9-query.scw PDB file : Tito_Scwrl_2EV9.pdb: