Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEVSRDMPLEDIVIETPITE-TTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGLYRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCLVAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK 1V9S Chain:B ((1-208)) -MRITLVDHPLVQHKLAHLRDKRTGPKDFRELAEEVAMLMAYEAMRDLELEETTVETPIAPARVKVL-SGKKLALVAILRAGLVMVEGILKLVPHARVGHIGLYRDPESLNPVQYYIKLPPDIAERRAFLLDPMLATGGSASLALSLLKERGATGVKLMAILAAPEGLERIAKDHPDTEVVVAAIDERLNDHGYIVPGLGDAGDRIYGTK

General information: TITO was launched using: RESULT: Template: 1V9S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1073 -87521 -81.57 -422.80 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.90 3D Compatibility (PKB) : -81.57 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.90 QMean score : 0.633

(partial model without unconserved sides chains): PDB file : Tito_1V9S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1V9S-query.scw PDB file : Tito_Scwrl_1V9S.pdb: