Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRYNSIAKVRKLIRRSVLLLKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQIDL---DLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLVTCYTPNSQSELKRLDYRLEWEEAFYNYLENL-KKQKPVIVCGDLNVAHQKIDLKNWKTNQKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYFLTSKDLEPGLADAKIHTDIMGSDHCPVELDLK 5J8N Chain:A ((2-256)) -----------------EHYNLISWNVNGLRAAVKKGFLDLLLEHRFDIVCVQETKVSQDKLPREVKNIQGYYNYFVSAEQNGYSGVGTFSKNKPIKLEKGMGIEVFDREGRFLRTDYEDFVLLNIYFPNGKMSQERLGYKMAFYDAFLDYANALKSEGKKLVICGDVNTAHKEIDLARPKQNEMISGFLPEERAWMDKFLAAGYLDSFRMFNPEG-GNYSWWSYRTGARSRNVGWRLDYVFVSENLRENVKSASIYPEIMGSDHCPVGLELE

General information: TITO was launched using: RESULT: Template: 5J8N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1363 2995 2.20 11.93 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : 2.20 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.584

(partial model without unconserved sides chains): PDB file : Tito_5J8N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5J8N-query.scw PDB file : Tito_Scwrl_5J8N.pdb: