Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKAVAYQKAGPITLPEALVDIELDTPVAKGHDLLIRVQAVSVNPVDTKIRKNVSAEQSGWKVLGWDAVGTVEAIGDKVTQFKIGDVVWYAGALNRQGSNSELQLVDERIVGHKPKTLEATEAAALPLTAITAWEMLFDRLQVPK--TAPANTTILVIGGAGGVGSITIQLLKQLTNLTIIATASRPETKEWVEQLGADYVLDHRQSLAAQIKLLGLSAPLYVFSTTETDQHLSDIVELIAPQGHFGLIDDPDQLDIKPFKSKSVSVHWEFMFTRSMFQTEDMEKQSALLNEVSKLVDEGKIKTTVTQ---VLSPINAENLTRVHEQIESGSTKGKIVLHGF 4DVJ Chain:B ((23-358)) MKAVGYNKPAPITDDASLLDIELPKPAPAGHDILVEVKAVSVNPVDYKVRRSTPPD-TDWKVIGYDAAGIVSAVGPDVTLFRPGDEVFYAGSIIRPGTNAEFHLVDERIVGRKPKTLDWAEAAALPLTSITAWEAFFDRLDVNKPVPGA-APAILIVGGAGGVGSIAVQIARQRTDLTVIATASRPETQEWVKSLGAHHVIDHSKPLAAEVAALGLGAPAFVFSTTHTDKHAAEIADLIAPQGRFCLIDDPSAFDIMLFKRKAVSIHHELMFTRPMFGTPDMSEQGRLLNDVSRLVDEGRLRTTLTNRLSPI-NA--ANLKQAHALVESGTARGKVVIEG-

General information: TITO was launched using: RESULT: Template: 4DVJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2074 -71267 -34.36 -215.96 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.89 3D Compatibility (PKB) : -34.36 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.573

(partial model without unconserved sides chains): PDB file : Tito_4DVJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DVJ-query.scw PDB file : Tito_Scwrl_4DVJ.pdb: