Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLVVPAEHPETVHNETDRVERILVVDDDVRLRTLLQRFLEDKGFVVKTAHDASQMDRLLQRELFSLIVLDFMLPVEDGLSICRRLRQSNIDTPIIMLTARGSDSDRIAGLEAGADDYLPKPFNPNELLARIRAVLRRQVREVPGAPSQQVEVVSFGPWSLDLSTRTLTREGQIVTLTTGEFAVLKALVQHPREPLTRDKLMNLARGREWGAMERSIDVQVSRLRRLIEDNPARARYIQTVWGVGYVFVPDGAE 1YS7 Chain:B ((7-232)) -------------------SPRVLVVDDDSDVLASLERGLRLSGFEVATAVDGAEALRSATENRPDAIVLDINMPVLDGVSVVTALRAMDNDVPVCVLSARSSVDDRVAGLEAGADDYLVKPFVLAELVARVKALLRRRGSTATS----SSETITVGPLEVDIPGRRARVNGVDVDLTKREFDLLAVLAEHKTAVLSRAQLLELVWGYDFAADTNVVDVFIGYLRRKLEAG-G-PRLLHTVRGVGFVLRM----

General information: TITO was launched using: RESULT: Template: 1YS7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1229 -154706 -125.88 -687.58 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -125.88 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_1YS7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YS7-query.scw PDB file : Tito_Scwrl_1YS7.pdb: