Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQVDIAALSPQVVWQHFQTLCTIPRPSKHEQQLREFLQNWAESRNLETYV----DEVGNLIIRKNATPGKKHVSGVILQGHLDMVTQANTGTVHDFF----KDPIRPVLEEGWLIAKDTTLGADNGIGVALALAVLDSND-----IHGPIEVLLTVDEEAG-----MSGARLLETGV---------LKGKWLFNIDTEEWGELYLGCAGSIDVEVEQPLNYEPIPENLNIVNIQVAGLKGGHSGV-----DIHLGRGNANVILARFLNQHLASLGGHLVEFTGGTARNALPREAVATIAISPNQLSSLEKLLAEYQAAWKEQLKGIDDNLQLSIQSTGAKVTEVINQQQQNEWLQALATSPYGVASMSQVLPDVVETSNNIGVVRLNREGGKAILMVRSMVNQEAQDFAEKIQAHFSQFNIGST--LTPLVSGWTPNPDSAALKCLQQAYQNAFNIEPNLK---VIHAGLECGIIAEHYPHLQMVSFGPDIQGAHAPGERVKVDTVEKCWKLLVTALASVE 5K8O Chain:D ((15-420)) -------DGMREGLIQTAVELGSIEAPTGREGAAGDYVYEWMARNGFGPERVGVFDDRFNVVGRLRGT---GGGASLSFNSHLDTIMARED--TARFADANDRIYHEAWHEEGRIYGYSV---VNCKGPMACWLIAAKALKEAGAALKGDVVLTAVCGEIDCEPVDEFQGHDYLAEDIGARYAISHGAISDYALVAEATN-FKPAWVE------------------AGKVFLKVTVFAGPSRYTPYVPRPVAA-LDSPNAIVRMAKLVEALEEW-ADNY---EKRY--T--RE-----YGGG----------TV-------------VPKVAI--GA-----------------------IRGGV-------------PYKIYRFP---ELCSIYMDIRLNPDTNPLVVQREVEAVVSKLGLKAEVKPFLFRRGYEAQGIEPLQNALEVAHREVVGRPTERPGSPECSMWRDTNPYNEL--GIPSLTYGCGG-GAGGGNTYFLVDDMLKAAKVYAMTAMDL-

General information: TITO was launched using: RESULT: Template: 5K8O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1943 -21599 -11.12 -58.53 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain D : 0.64 3D Compatibility (PKB) : -11.12 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.409

(partial model without unconserved sides chains): PDB file : Tito_5K8O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5K8O-query.scw PDB file : Tito_Scwrl_5K8O.pdb: