Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MECSMNLADRDGFIWQDGQLIDWRDAKIHVLTHTLHYSMGVFEGVRAYETPKGTAIFRLQDHTKRLLNSAKIYQMKVPYDQAALEQAQIDVVRENKLASCYLRPLIWIGSEKLGIAATN-NTIHAAVAAWAWGAYLGDEAMAKGIRVKTSSFTHHHPNVTMCKAKASGNYTLSILAHQEVAHSGYDEAMLMDPQGYVCQGSGENVFLIKDGVLHTPDIAGGALDGITRQTVMTIAKDLGYEVVERRITRDEFYIADEAFFTGTAAEVTPIREYDDRQIGAGCRGPITTEIQKTFFDAVQGKKPKYEHWLTYVK 5E25 Chain:A ((2-287)) ----------ELLVYMNGEFVPESQAKVSVFDHGFLYGDGVFEGIRAYNGK----VFKLYEHIDRLYDCARVIDLKIPLSKEEFAEAILETLRRNNLRDAYIRPIVTRGAGDLGLDPRKCPSPNVIIITKPWGKLYG-DLYEKGLKAITVAIRRNAIDSLPPNIK-SLNYLNNILAKIEANAKGGDEAIFLDHNGYISEGSGDNIFIVKNGTITTPPTL-NNLKGITRQVVIELINELEIPFREANIGLFDLYSADEIFVTGTAAEIAPVTYIDGRTVGNGKPGKVTKMLMEKFRERTENEGV----------

General information: TITO was launched using: RESULT: Template: 5E25.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1545 -49167 -31.82 -172.51 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -31.82 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.454

(partial model without unconserved sides chains): PDB file : Tito_5E25.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5E25-query.scw PDB file : Tito_Scwrl_5E25.pdb: