Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGMSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGAGKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVLENIQLP---LELAGVPKSKRKERAEELLR--------LVGLEGRGQAYPSQLSGGQKQRVGIARALANDPEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMELGKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKEHPSGRLATFDL 2FF7 Chain:A ((25-235)) -------------------------LDNINLSIKQGEVIGIVGRSGSGKSTLTKLIQRFYIPENGQVLIDGHDLALADPNWLR---RQVGVVLQD-NVLLNRSIIDNISLANPGMSVEKVIYAAKLAGAHDFISELREGYNTIVGEQGAG------LSGGQRQRIAIARALVNNPKILIFDEATSALDYESEHVIMRNMHKICK--GRTVIIIAHRLSTVKN-ADRIIVMEKGKIVEQGKHKELLSEPE---------------------------------------

General information: TITO was launched using: RESULT: Template: 2FF7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 948 -27534 -29.04 -137.67 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -29.04 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.521

(partial model without unconserved sides chains): PDB file : Tito_2FF7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FF7-query.scw PDB file : Tito_Scwrl_2FF7.pdb: