Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIGTQNVLKCLERCDRLDIPVYQGMEKPLKQPF-ISAQDTHGMDGLGETNFPMILR-KQAEPLHAVDFLADYFKEK-T--N--TSVIALGPLTNIASALKVNPNIGKHM-----ERFVSMGGTYKSHGNCSPVAEYNYWCDPEAASYVFENLK--QTIEMVGLDVTREIVLTPTILEYCCQMN--------------PEEGEYLKAITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICE-GFKSFTAVETQGI-SRGQTLVDRYEF------WQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG 3FZ0 Chain:B ((6-359)) HRKLIIDTDCGGDDAIAIMLAMTQPDVEVIAITVVWGNVEVNQGMENIGKLLDLYDA-DIPFFRGAEGPLVGERETVQWGGFGSDGFGDAGFPPSQRVALQPKRHAALEILKILEEAEPSDDVVYQLVALGPLTNVALALRLNPDLFSKLGTDTIPGIVIMNGTSESKGNSNMAAEFNSHCDPEAGVVVLQHKGWKCPVQLVNWEVTVNSPMTWGF---YDKLVNRESTPNGRVAVNQNKWQEFIEKLFQRLEAFTR-----VTCVVPDAVAVLVAIRPESVLDSFLTYVTVELHGRETRGATCIDWYGTEQSMAKKGRWRNCNVITKVDNEMFLKALRDIVEYV---------------

General information: TITO was launched using: RESULT: Template: 3FZ0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1798 -51125 -28.43 -170.42 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -28.43 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.411

(partial model without unconserved sides chains): PDB file : Tito_3FZ0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FZ0-query.scw PDB file : Tito_Scwrl_3FZ0.pdb: