Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKGSIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDIIDTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDYKENYRNLPYIGVLKEEVYSN 4RHT Chain:D ((21-197)) ----DIKSVLLTAEQIQARIAELGEQIGNDY--QDLLLITVLKGAVLFVTDLARAIPVPTQFEFMAVSSYGS----SGVVRILKDLDRDIHGRDVLIVEDVVDSGLTLSWLSRNLTSRNPRSLRVCTLLRKPD---ANVEIAYVGFDIPNDFVVGYGLDYDERYRDLSYIGTLDP-----

General information: TITO was launched using: RESULT: Template: 4RHT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 774 -129262 -167.00 -797.91 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain D : 0.84 3D Compatibility (PKB) : -167.00 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.559

(partial model without unconserved sides chains): PDB file : Tito_4RHT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RHT-query.scw PDB file : Tito_Scwrl_4RHT.pdb: