Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCRLRERTRMTQQDFRTKVDNTVFGVRATALIVQ-NHKLLVTKD-KGKYYTIGGAIQVNEKTEDAVVREVREELGVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDAPLTMQEDEKSQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE 1VCD Chain:A ((2-125)) ------------------------ELGAGGVVFNAKREVLLLRDRMGFWVFPKGHPEPGESLEEAAVREVWEETGVRAEVLLPLYPTRYVNP---KGVEREVHWFLMRGEGAP----RLEEGMTGAGWFSPEEARALLAF-PEDLGLLEVALERLP------

General information: TITO was launched using: RESULT: Template: 1VCD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 594 9485 15.97 77.75 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 15.97 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.377

(partial model without unconserved sides chains): PDB file : Tito_1VCD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VCD-query.scw PDB file : Tito_Scwrl_1VCD.pdb: