Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLDHHTDGILLAGTTAESPTLTHDEELELFAAVQKIVNGRVPLIAGVGTNDTRDSIEFVKEVAEFGGFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLADHPNIIGVKECTSLANMAYLIEHKPE----EFLVYTGEDGDAFHAMNLGADGVISVASHTNGDEMHEMFIAIAESDVKKAAAIQRKFIPKVNALFSYPS----PAPVKAVLNYMGFEAGPTRLPLVPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY 3DAQ Chain:D ((10-279)) -------------ALTTPF-TNNKVNLEALKAHVNFLLENNAQAIIVNGTTAESPTLTTDEKELILKTVIDLVDKRVPVIAGTGTNDTEKSIQASIQAKALGADAIML-ITPYYNKTNQRGLVKHFEAIADAVKLPVVLYNVPSRTNMTIEPETVEILSQHPYIVALKDATN--DFEYLEEVKKRIDTNSFALYSGNDDNVVEYYQRGGQGVISVIANVIPKEFQALYDA-----QQSGLDIQDQFKP-IGTLLSALSVDINPIPIKALTSYLGFGNYELRLPLVSLEDTDTK--------------------------

General information: TITO was launched using: RESULT: Template: 3DAQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1420 -95431 -67.20 -364.24 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain D : 0.78 3D Compatibility (PKB) : -67.20 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.518

(partial model without unconserved sides chains): PDB file : Tito_3DAQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DAQ-query.scw PDB file : Tito_Scwrl_3DAQ.pdb: