Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSVNQPKDYASIDAAPEERERGITINTAHVE--------------YETEK--------------RHYAHIDAPGHADYVKNMITGAAQMDGAILVVASTDG-PMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDLLSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERD-TDKPLLLPVEDVFSITGRGT--------VASGRIDRGIVKVNDEIEIV-GIKEETQKAVVTGVEMFRKQLDEGLAGDNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQFYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGMVTEIEA 4RCZ Chain:A ((3-267)) -----WPKVQPEVNIGVVGHVDHGKTTLVQAITGIWTSK----------------HSEELKRGMTIKLGYAETNIGVCESCKKPEAYVTEPSCKSCGSDDEPKFLRRISFIDAPGHEVLMATMLSGAALMDGAILVVAANEPFPQPQTREHFVALGIIGVKNLIIVQNKVDVVSKEEALSQYR-QIKQFTK--GTWAENVPIIPVSALHKINIDS--------LIEGIEEYIKTPYRDLSQKPVMLVIRS-FDVNKPGTQFNELKGGVIGGSIIQGLFKVDQEIKVLPGLRVEKQGKV-------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4RCZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1124 5494 4.89 24.31 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 4.89 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.480

(partial model without unconserved sides chains): PDB file : Tito_4RCZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RCZ-query.scw PDB file : Tito_Scwrl_4RCZ.pdb: