Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEKESVDIAILDVEMPVKTGLEVLEWIRSEKLETKVVVVTTFKRAGYFERAVKAGVDAYVLKERSIADLMQTLHTVLEGRKEYSPELM---------EMVMTRPNPLTEQEIAVLKGIARGLSNQEIADQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL 5HEV Chain:C ((2-209)) IKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAMALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYLLKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLISEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLV

General information: TITO was launched using: RESULT: Template: 5HEV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 870 -77250 -88.79 -388.19 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain C : 0.76 3D Compatibility (PKB) : -88.79 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.584

(partial model without unconserved sides chains): PDB file : Tito_5HEV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HEV-query.scw PDB file : Tito_Scwrl_5HEV.pdb: