Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIRGFELVSSFTD----EN-LLPKRETAHAAGYDLKVA--VRTVVAPGEIVLVPTGVKAYMQP-TEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQMKNITDQEVVLEVGERIVQAVFATFLIADGDAAD---GVRTGGFGSTGH 1EUW Chain:A ((3-136)) -----KKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLNDAVELAPGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQ------GQLMISVWNRGQDSFTIQPGERIAQMIFVPVVQAE-FNLVEDF------------

General information: TITO was launched using: RESULT: Template: 1EUW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 585 -30856 -52.74 -250.86 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -52.74 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.385

(partial model without unconserved sides chains): PDB file : Tito_1EUW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EUW-query.scw PDB file : Tito_Scwrl_1EUW.pdb: