Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDQLQIKDLEMFAYHGLFPSEKELGQKFIVSAILSYDMTKAATDLDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWAPVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRAR-GIHILKESSVLATEPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVRE-VHWGPRLIDLDLLFVEDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK 2QX0 Chain:B ((1-152)) ----------------------------------------------------------------------------------------------------------------------MIRVYIALGSNLAMPLQQVSAAREALAHLPRSRLVACSPLYRTKPLGPQDQPDFLNAVVALDTSLPPEQLLDHTQAIERNQGRVRKEQRWGPRTLDLDIMLYGDQVIKTDRLTIPHYGLKAREFMLYPLADIAPDLIFPD-GESLSECLKRVD-

General information: TITO was launched using: RESULT: Template: 2QX0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 720 -6923 -9.62 -46.15 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -9.62 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.547

(partial model without unconserved sides chains): PDB file : Tito_2QX0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QX0-query.scw PDB file : Tito_Scwrl_2QX0.pdb: