Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEELIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTIRGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF 3NGU Chain:B ((4-133)) -ERTFIAVKPDGVQRGLVGEIIARFERKGYKLVALKIL-QPTTEQAQGHYKDLCSKPFFPALVKYFSSGPIVCMVWEGKNVVKSGRVLLGATNPADSQPGTIRGDFAVDVG-----RNVCHGSDSVESAEREIAFWF

General information: TITO was launched using: RESULT: Template: 3NGU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 608 -69450 -114.23 -534.23 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -114.23 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.559

(partial model without unconserved sides chains): PDB file : Tito_3NGU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3NGU-query.scw PDB file : Tito_Scwrl_3NGU.pdb: