Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQQQVIIVGGGMVGLSLSLMLAKANIAVKLLEAVKYPNYDDQNVAPYHSSFDARNTALSRRSVQIYQKLGLWDALQQHATPILQVHITEQGSFGKARLIAEQEKVESFGQ-------VIENAWLGRVLLTQVRQQPLIELIDGVQVTALTQDAEQVHIEAQRGDEVLKLESKLLIAADGRDSFCRQAIGVGVDV--HDYDQVAIVTTVQ-TSKPHEHVGFE----RFSALGPLALLPLPGEYRRSVV-------WPVKKGTEGEWLGEENDQHFLDALQKTYGDRAGKFEKTGKRFSYPLSQVLAHKQAV-----GRIILMGNAAHTIHPVAGQGFNLCLRDADVLLRYLVNQLSASDDIGNPDNLLAYEEARLSDQQRVIKFCDTVVRGFSN--QNPLLKLIRNTGLIAFDVIPG----VKPLVAN----YAMGLKA---------------------------------------- 5FN0 Chain:A ((6-460)) NARQVTIIGAGLAGTLVARLLARNGWQVNLFERRPDPRIETG------ARGRSINLALAERGAHALRLAGLEREVLAEAVMMRGRMVHVPG---------TPPNLQPYGRDDSEVIWSINRDRLNRILLDGAEAAG-ASIHFNLGLDSVDFARQRLTLSNVSG-ERLEKRFHLLIGADGCNSAVRQAMASVVDLGEHLETQPHGYKELQITPEASAQFNLEPNALHIWPHGDYMCIALPNLDRSFTVTLFLHHQSPAAQPASPSFAQLVDGHAARRFFQRQFPDLSPMLDSLEQDFEHHPTGKLATLRLTTWHVGGQAVLLGDAAHPMVPFHGQGMNCALEDAVALAEHLQSAAD------NASALAAFTAQRQPDALAIQAMALENYVEMSSKVASPTYLLERELGQIMAQRQPTRFIPRYSMVTFSRLPYAQAMARGQIQEQLLKFAVANHSDLTSINLDAVEHEVTRCLPPLSHL

General information: TITO was launched using: RESULT: Template: 5FN0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2135 -49206 -23.05 -129.83 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -23.05 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.315

(partial model without unconserved sides chains): PDB file : Tito_5FN0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FN0-query.scw PDB file : Tito_Scwrl_5FN0.pdb: