Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGARRVPKYFPSMA 3VIG Chain:A ((26-174)) -------QIEGAWDENGKGPNIWDTLTHEHPDYVVDGA-----TGDIAD---------DSYHLYKEDVKILKELGAQVYRFSISWARVLPEGHDNIVNQDGIDYYNNLINELLANGIEPMVTMYHWDLPQAL-QDLGGWPNLVLAKYSENYARVLFKNFGDRVKLWLTFNE-------------------------------

General information: TITO was launched using: RESULT: Template: 3VIG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 634 -46787 -73.80 -314.00 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -73.80 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.517

(partial model without unconserved sides chains): PDB file : Tito_3VIG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3VIG-query.scw PDB file : Tito_Scwrl_3VIG.pdb: