Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MERFDVERMIQMKIFVVGANGQIGRHLIKDLAPSS--HEIFAGVRDVASQTLVKKENVSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSRYLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ--------LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSK-ELNTAFQEWAKEE 2JL1 Chain:A ((1-214)) -----------FSIAVTGATGQLGGLVIQHLLKKVPASQIIAIVRNVEKASTLADQGVEVRHGDYN-QPESLQKAFAGVSKLLFISGPHYD--NTLLIVQHANVVKAARDAGVKHIAYTGYAFAEES------IIPLAHVHLATEYAIRTTNIPYTFLRNALYTDFFVNEGLRASTESGAIVTNAGSGIVNSVTRNELALAAATVLTEEGHENKTYNLVSNQPWTFDELAQILS---

General information: TITO was launched using: RESULT: Template: 2JL1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 985 -39988 -40.60 -196.98 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -40.60 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.478

(partial model without unconserved sides chains): PDB file : Tito_2JL1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JL1-query.scw PDB file : Tito_Scwrl_2JL1.pdb: