Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRIILLGPPGAGKGTQAQLICKRYNIPQISTGDMLRAAIREGTELGLKAKSVMESGGLVSDELIIGLVKERIAQPDCVNGCIFDGFPRTIPQAEA----LEKEGISIDHVIEIDVPDEEIVKRLSGRRQHPASGRVYHVVYNPPKVEGKDDETGEDLVQRPDDQEETIRKRLASYHTETEQLVGFYQGRAASGENAPTYDKLDGLRTIEDVQKDLFNILDK 4CF7 Chain:B ((1-202)) MILVFLGPPGAGKGTQAKRLAKEKGFVHISTGDILREAVQKGTPLGKKAKEYMERGELVPDDLIIALIEEVFPKHGNV---IFDGFPRTVKQAEALDEMLEKKGLKVDHVLLFEVPDEVVIERLSGRRINPETGEVYHVKYNPPP-------PGVKVIQREDDKPEVIKKRLEVYREQTAPLIEYYKKKG-------ILRIIDASKPVEEVYRQVLEVI--

General information: TITO was launched using: RESULT: Template: 4CF7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 920 -33230 -36.12 -167.83 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.86 3D Compatibility (PKB) : -36.12 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.478

(partial model without unconserved sides chains): PDB file : Tito_4CF7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CF7-query.scw PDB file : Tito_Scwrl_4CF7.pdb: