Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIILAIIQDKDSNRLSNELIDANIR-ATKLSSTGGF--------L---------KAGNSTFIVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLPVEGFHQF 4USJ Chain:C ((18-119)) FFRIEAIFRPWRLPFVIDTLSKYGIRGLTNTPVKGVGVQGGSRERYAGTEFGPSNLVDKEKLDIVVSRAQVDAVVRLVAASAYTG------------------------EIGDGKIFVHPVAEVVR-

General information: TITO was launched using: RESULT: Template: 4USJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 288 -22849 -79.34 -272.01 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain C : 0.63 3D Compatibility (PKB) : -79.34 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.741

(partial model without unconserved sides chains): PDB file : Tito_4USJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4USJ-query.scw PDB file : Tito_Scwrl_4USJ.pdb: