Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGRKTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAVYQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQRNENPVKSGTGELTI 3FY8 Chain:X ((2-151)) -LSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMGRKTFESIG-KPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLP----GHVFIFGGQTLYEEMIDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTF-------------------

General information: TITO was launched using: RESULT: Template: 3FY8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 687 -113031 -164.53 -753.54 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain X : 0.84 3D Compatibility (PKB) : -164.53 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.521

(partial model without unconserved sides chains): PDB file : Tito_3FY8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FY8-query.scw PDB file : Tito_Scwrl_3FY8.pdb: