Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEKDIERILISQEEIQVRCKELGKELTEIYQN-----TNPL-VVGVLKGAVPFMADIVRSI---DTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDIIDSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDYAEAYRNLPYIGVLKPSVYQSN 1PZM Chain:B ((25-201)) -------RTLVTQEQVWAATAKCAKKIAADYKDFHLTADNPLYLLCVLKGSFIFTADLARFLADEGVPVKVEFICA------------VRMLLDVRDSVENRHIMLVEDIVDSAITLQYLMRFMLAKKPASLKTVVLLDKPSGRKVDVLVDYPVITIPRAFVIGYGMDFAESYRELRDICVLKK------

General information: TITO was launched using: RESULT: Template: 1PZM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 719 -20947 -29.13 -134.28 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -29.13 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.476

(partial model without unconserved sides chains): PDB file : Tito_1PZM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PZM-query.scw PDB file : Tito_Scwrl_1PZM.pdb: