Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIRGFELVSSFTDEN-LLPKRETAHAAGYDLKVAVRTVVAPGEIVLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQMKNITDQEVVLEVGERIVQAVFATFLIADGDAAD-----GVRT-GGFGSTGH 4OOP Chain:B ((25-166)) ---PSPFFKVKKLSEKAVIPTRGSPLSAGYDLSSAVDSKVPARGKALIPTDLSIAVPEGTYARIAPRSGLAWKHSIDV--GAGVIDADYR------GPVGVILFNHSDADFEVKFGDRIAQLIIEKIVTPD-VVEVDDLDETVRGDGGFGSTGV

General information: TITO was launched using: RESULT: Template: 4OOP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 582 -3253 -5.59 -24.09 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : -5.59 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.448

(partial model without unconserved sides chains): PDB file : Tito_4OOP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OOP-query.scw PDB file : Tito_Scwrl_4OOP.pdb: