Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVQVKLLDPRLGKEWPLPSYATAGSAGLDLRACLDEAIEIEPGQTVLVKTGMAIYIHDVNFAGLILPRSGLGHKHGIVLGNLVGLIDSDYQGELMVSVWNRGQTTFRLEPGERLAQYVLVPVV-QAEFEQVEEFEETLRGAGGFGHTGKQ 3F4F Chain:B ((40-163)) -----------------VPTKGSATAAGYDIYASQD--ITIPAMGQGMVSTDISFTVP-VGTYGRIAPRSGLAVKNGIQTG--AGVVDRDYTGEVKVVLFNHSQRDFAIKKGDRVAQLILEKIVDDAQIVVVDSLEESARGAGGFG-----

General information: TITO was launched using: RESULT: Template: 3F4F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 479 -61804 -129.03 -502.47 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : -129.03 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.421

(partial model without unconserved sides chains): PDB file : Tito_3F4F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3F4F-query.scw PDB file : Tito_Scwrl_3F4F.pdb: