Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTFIKLMYREEKASKGTLNVAGHDL-IAIKNREVPYLRREIGVVFQDYKLLPRKTV-----------YENVAYAMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLIADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRDQAEGEYGYDD 2FGJ Chain:A ((27-222)) -------------------NINLSIKQGEVIGIVGRSGSGKSTLTKLIQRFYIPENGQVLIDGHDLALADPN----WLRRQVGVVLQDNVLLNRSIIDNISLANPGMSVEKVIYAAKLAG--AHDFISELRE-----GYNTIVGEQGAGLSGGQRQRIAIARALVNNPKILIFDEATSALDYESEHVIMRNMHKICKGRTVIIIAARLSTVKNA--DRIIVMEKGKIV------------

General information: TITO was launched using: RESULT: Template: 2FGJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 821 -24187 -29.46 -131.45 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -29.46 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_2FGJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FGJ-query.scw PDB file : Tito_Scwrl_2FGJ.pdb: